Cheese has always been one of the most important dairy products worldwide, holding a special place in nutrition, culture, and the economy of many countries. Among the wide variety of cheeses, Asiago PDO from Italy stands out as one of the most popular and widely consumed types. Known for its distinctive flavor, unique aroma, and high nutritional value, Asiago is enjoyed not only in Italy but across the globe. A key feature of this cheese is the use of starter cultures during production, which play a decisive role in shaping its flavor, texture, and bioactive properties.
History and Cultural Significance
The roots of Asiago cheese trace back centuries to the mountainous region of the same name in northern Italy. This area, with its lush pastures, has long provided ideal conditions for cow’s milk production. Historically, local herders used fresh cow’s milk to produce a cheese that could last through harsh winters while serving as a valuable food source. Over time, traditional methods were refined, and today, Asiago is recognized in Europe with the Protected Designation of Origin (PDO) label. This certification ensures that the cheese is produced only in its designated region under traditional standards.
Raw Materials and Production Process
Like many traditional cheeses, Asiago relies on a few essential ingredients: fresh cow’s milk, rennet (an enzyme used for coagulation), salt, and starter cultures. The production involves several key steps:
- Coagulation: After adding rennet and starter cultures, milk proteins—mainly caseins—aggregate and form the Curd, while the liquid Whey separates.
- Cutting: The Curd is cut into smaller pieces with special tools. The size of the cuts strongly influences the final moisture content; the smaller the Curd, the drier and firmer the cheese.
- Heating & Stirring: The Curds are gradually heated and stirred, which drives out more Whey and strengthens the texture. This step is critical in creating Asiago’s elastic yet firm consistency.
- Moulding & Pressing: The prepared Curds are placed into molds and pressed mechanically. This shapes the cheese, removes excess Whey, and produces a compact structure.
- Salting: The molded cheeses are either immersed in brine or dry-salted. Salt contributes to flavor while also inhibiting undesirable microbial growth and extending shelf life.
- Ripening: The cheeses are aged in controlled conditions for weeks or months. During this period, microbial and enzymatic activities develop complex nutty, buttery, or slightly piquant flavors, and the texture evolves from soft to firmer and grainier.

Role of Starter Cultures (SLAB and NSLAB)
One of the most important elements in Asiago production is the use of starter cultures. These are groups of lactic acid bacteria added to milk to initiate lactose fermentation. The result is the production of lactic acid, which lowers milk pH, improves coagulation, and creates favorable conditions for cheese ripening.
Traditionally, Asiago is made with Natural Whey Starters (NWS), carried over from previous cheesemaking batches. These cultures are complex mixtures of bacterial strains, giving Asiago its distinctive and authentic flavor.
Starter Lactic Acid Bacteria (SLAB): Thermophilic and mesophilic species such as Streptococcus, Lactobacillus, and Lactococcus. They are added at the beginning and are responsible for rapid lactose acidification, ensuring strong coagulation, controlling unwanted microbes, and setting the stage for ripening.
Non-Starter Lactic Acid Bacteria (NSLAB): These include various lactobacilli and related species that grow during ripening. They break down proteins through proteolysis, producing peptides and organic acids that contribute to complex flavors and bioactive properties, such as ACE-inhibitory activity and antioxidant effects.
In practice, SLAB species such as Streptococcus thermophilus rapidly acidify milk, while NSLAB, particularly lactobacilli, drive flavor development by releasing peptides that influence both taste and health. Industrial starter cultures offer consistency and predictable results with higher peptide production, while natural whey starters provide microbial diversity and unique sensory characteristics.
Practical Implications for Cheesemaking
Producers aiming for consistent quality and repeatable outcomes often prefer industrial starter cultures. These guarantee uniform acidification and controlled ripening. On the other hand, producers seeking distinctive, region-specific flavors often rely on natural whey starters, though these require stricter microbial monitoring. In both cases, tracking microbial activity and proteolysis throughout ripening is key to achieving a stable, high-quality product.
Influence of Starter Cultures on Flavor, Texture, and Quality
Starter cultures remain active throughout ripening, breaking down proteins and fats into aromatic compounds that define Asiago’s identity.
Flavor: Organic acids, alcohols, and esters produced by bacteria generate Asiago’s nutty, buttery, and sometimes slightly sharp notes.
Texture: Enzymatic activity gradually transforms the Curds, shifting the cheese from soft and elastic to firmer and grainier.
Health: Some starter bacteria produce bioactive peptides with antimicrobial or antioxidant properties, enhancing the nutritional value of the cheese.
Nutritional and Health Aspects
Beyond its sensory appeal, Asiago is a rich source of protein, calcium, and vitamins. The presence of beneficial lactic acid bacteria supports gut health. Studies suggest that regular consumption of traditional cheeses like Asiago may improve digestion, strengthen the immune system, and even reduce the risk of certain metabolic disorders.
Future Perspectives
With growing global interest in natural and functional foods, cheeses like Asiago are gaining attention. Innovations in starter culture selection and optimization may lead to products with enhanced flavor, improved consistency, and even added health benefits. Nevertheless, preserving traditional practices and regional identity remains essential to maintaining Asiago’s authenticity.
Conclusion
Asiago cheese represents a harmonious blend of tradition and science. Rooted in Italian history and culture, it has evolved into a product celebrated for its distinctive flavor and nutritional value, thanks largely to the pivotal role of starter cultures. A deeper understanding of these microbial communities not only improves cheese quality but also inspires further innovation in the dairy industry. Asiago clearly demonstrates that even the smallest microbes can have the most powerful impact on our food.
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